2021年YGM003A系列酵母选择营养缺陷培养基-& Ura Minus Media
酵母培养基配制
Yeast media preparation
----------------------------------------------------------------------------------------------------------------------
【液体培养基】 8g粉状培养基泛基诺目录号YGM0003A系列选择培养基加950 ml 水,常规高压灭菌后再加50ml 40%过滤除菌的无菌葡萄糖。（备注： YPD、YPDA和YPD plus这三种系列不需要再加糖，直接按瓶子上标签指示加水配制即可）。配制液体培养基不需要调pH，因为酵母偏好酸性条件生长。如果为了实验方便，葡萄糖也可按2%的量以粉末的形式加进去高压灭菌（见下面标准配制法注解）。
【固体培养基】8g粉状培养基(泛基诺目录号:YGM0003A系列)加950 ml, 用NaOH调pH至大致范围5.8~6.5, 然后按20g/L加琼脂粉, 高压灭菌后再加预温的50ml 40%无菌葡萄糖, 摇匀后倾倒平皿(备注:如不调pH, 琼脂在酸性条件下会产生崩解)。如果为了实验方便，葡萄糖也可按2%的量以粉末的形式加进去高压灭菌。
【标准配制法】标准流程要求葡萄糖过滤除菌，因为葡萄糖与培养基中的氨基酸在高温高压下发生化学反应。但有些实验者为方便起见，将固体葡萄糖按2% 的量(按每升20克)直接加到培养基后一起高压灭菌，虽然酵母也可以生长，但一般不推荐这种将葡萄糖高压灭菌的方法。如果实在要图省事想用这种方法，那么一定要控制好高压时间在18-20分钟结束，待压力下降和温度降到安全范围后应尽快取出。否则液体颜色会变成黄色而对酵母特性发生不可预知的影响。但半乳糖和棉子糖不能高压灭菌，只能用过滤除菌制备，建议制备高浓度40%的储存液，按计算好的体积加到高压灭菌的培养基使得终浓度为2%。40％无菌棉子糖和半乳糖可以通过当地经销商从泛基诺公司订购；如客户自行配制高浓度半乳糖时发现很难溶解，可通过QQ技术咨询，实时在线工作QQ号：2827505022
【说明】用于组成型表达（如酵母双杂交、酵母单杂交）以及其它采用组成型启动子的实验一般都加葡萄糖；诱导型表达如采用GAL1启动子载体的表达一般都加半乳糖和棉子糖。葡萄糖可以勉强高压灭菌，但半乳糖和棉子糖是绝对不能高压灭菌的, 应采用无菌过滤法先制备40%的无菌储备液，待不含糖的培养基高压灭菌后按最终应用浓度为2％ 的量计算需要加入的无菌液体糖储存液：
【举例】如配制1升培养基则称取8克培养基加水950 ml ，常规高压灭菌（121度20分钟）完成后待温度降至安全范围70度时趁热立刻加入50ml 40%过滤除菌的无菌糖，充分混匀。
酵母转化即用储存液 (Stock Solutions):
10 x TE： YGM022D Sterilized
10x LiAc pH 7.5： YGM022A Sterilized
50% PEG 4000： YGM022B Sterilized
Carrier DNA(10mg/ml)： YGM022C Sterilized
注: 1. 50% PEG不要高压灭菌（高压导致PEG分子断裂）, 应过滤除菌, 否则会影响转化效率
2. 50% PEG很难过滤, 建议使用本公司即用型储液(目录号:YGM022B)
北京泛基诺 (FunGenome) 科技有限公司
网址：www.fungenome.com 技术支持QQ:2827505022
2021年YGM003A-X系列酵母选择营养缺陷培养基
Yeast selective media or selective minus media
|
货号 |
品名 |
包装 |
价格 |
|
YGM003A-1 |
SC Complete medium |
40g(8g/L) |
630.00 |
|
YGM003A-2 |
-Trp |
40g(8g/L) |
630.00 |
|
YGM003A-3 |
-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-4 |
-His |
40g(8g/L) |
630.00 |
|
YGM003A-5 |
-Leu |
40g(8g/L) |
630.00 |
|
YGM003A-6 |
-His-Leu |
40g(8g/L) |
630.00 |
|
YGM003A-7 |
-Trp-Leu |
40g(8g/L) |
630.00 |
|
YGM003A-8 |
-Trp-Leu-His |
40g(8g/L) |
630.00 |
|
YGM003A-9 |
-Trp-Leu-His-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-10 |
-Trp-Leu-His-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-11 |
-Trp-Leu-His-Ura-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-12 |
-Trp-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-13 |
-Ura-His |
40g(8g/L) |
630.00 |
|
YGM003A-14 |
-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-15 |
-Trp-His-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-16 |
-Leu-His-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-17 |
-Trp-His |
40g(8g/L) |
630.00 |
|
YGM003A-18 |
-Trp-His-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-19 |
-Trp-Leu-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-20 |
-Trp-Leu-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-21 |
-Leu-His-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-22 |
-Ura-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-23 |
-His-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-24 |
-Leu-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-31 |
-Trp-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-32 |
-Trp-Ura-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-35 |
-Leu-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-36 |
-Leu-Ura-Ade |
40g(8g/L) |
630.00 |
|
YGM003A-40 |
-Trp-Leu-His-Ade-Ura |
40g(8g/L) |
630.00 |
|
YGM003A-50 |
YPD |
250g(50g/L) |
520.00 |
|
YGM003A-51 |
YPD plus |
250g(50g/L) |
520.00 |
|
YGM003A-52 |
YPDA |
250g(50g/L) |
520.00 |
|
YGM003A-60 |
-Trp-Leu-His-Ade-Ura-Met |
40g(8g/L) |
650.00 |
|
YGM003A-60Lys |
-Trp-Leu-His-Ade-Ura-Met-Lys |
40g(8g/L) |
650.00 |
|
YGM003A-61 |
-Met |
40g(8g/L) |
650.00 |
|
YGM003A-61T |
-Met-Trp |
40g(8g/L) |
650.00 |
|
YGM003A-61L |
-Met-Leu |
40g(8g/L) |
650.00 |
|
YGM003A-61H |
-Met-His |
40g(8g/L) |
650.00 |
|
YGM003A-61A |
-Met-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-61U |
-Met-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-61LC |
-Met-Leu-Cys |
40g(8g/L) |
750.00 |
|
YGM003A-62UC |
-Met-Ura-Cys |
40g(8g/L |
750.00 |
|
YGM003A-62TL |
-Met-Trp-Leu |
40g(8g/L) |
650.00 |
|
YGM003A-62TH |
-Met-Trp-His |
40g(8g/L) |
650.00 |
|
YGM003A-62TA |
-Met-Trp-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-62TU |
-Met-Trp-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-62LH |
-Met-Leu-His |
40g(8g/L) |
650.00 |
|
YGM003A-62LA |
-Met-Leu-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-62LU |
-Met-Leu-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-62UA |
-Met-Ura-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-63TLH |
-Met-Trp-Leu-His |
40g(8g/L) |
650.00 |
|
YGM003A-63TLA |
-Met-Trp-Leu-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-63LHA |
-Met-Leu-His-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-63LHU |
-Met-Leu-His-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-63HAU |
-Met-His-Ade-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-64TLHA |
-Met-Trp-Leu-His-Ade |
40g(8g/L) |
650.00 |
|
YGM003A-64TLHU |
-Met-Trp-Leu-His-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-64TLAU |
-Met-Trp-Leu-Ade-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-64LHAU |
-Met-Leu-His-Ade-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-64THAU |
-Met-Trp-His-Ade-Ura |
40g(8g/L) |
650.00 |
|
YGM003A-65 |
-Lys |
40g(8g/L) |
750.00 |
|
YGM003A-66 |
-Arg |
40g(8g/L) |
750.00 |
|
YGM003A-67 |
-Ser |
40g(8g/L) |
750.00 |
|
YGM003A-68 |
-Thr |
40g(8g/L) |
750.00 |
|
YGM003A-69 |
-Lys-Arg-Ser-Thr |
40g(8g/L) |
750.00 |
|
YGM003A-70 |
-Arg-Lys |
40g(8g/L) |
750.00 |
|
YGM003A-71 |
-Ser-Thr |
40g(8g/L) |
750.00 |
|
YGM003A-72 |
-Arg-His-Leu-Ura |
40g(8g/L) |
750.00 |
|
YGM003A-73 |
-Trp-Lys |
40g(8g/L) |
750.00 |
|
YGM003A-74 |
-Arg-His-Leu |
40g(8g/L) |
750.00 |
|
YGM003A-75 |
-Arg-Leu-Ura |
40g(8g/L) |
750.00 |
|
YGM003A-76 |
-Lys-Ura |
40g(8g/L) |
750.00 |
|
YGM003A-77 |
-Ile-Val |
40g(8g/L) |
750.00 |
|
YGM003A-78 |
-Val |
40g(8g/L) |
750.00 |
|
YGM003A-79 |
-Ile |
40g(8g/L) |
750.00 |
|
YGM003A-80 |
-Ile-Val-Leu |
40g(8g/L) |
750.00 |
|
YGM003A-81 |
-Tyr |
40g(8g/L) |
750.00 |
|
YGM003A-82 |
-Asp |
40g(8g/L) |
750.00 |
|
YGM003A-83 |
-Phe |
40g(8g/L) |
750.00 |
|
YGM003A-84 |
-Arg-Ura-His |
40g(8g/L) |
750.00 |
|
YGM003A-85 |
-Arg-Leu |
40g(8g/L) |
750.00 |
|
YGM003A-86 |
-Ser-Trp-Leu |
40g(8g/L) |
750.00 |
|
YGM003A-87 |
-Lys-Leu-Ura |
40g(8g/L) |
750.00 |
|
YGM003A-88 |
-Lys-Arg-Leu-Ura-His |
40g(8g/L) |
750.00 |
|
YGM003A-89 |
-Lys-Leu-Ura-His |
40g(8g/L) |
750.00 |
|
YGM003A-90 |
-Lys-His |
40g(8g/L) |
750.00 |
|
YGM003A-91 |
-Ser-Trp-Leu-Ade |
40g(8g/L) |
750.00 |
|
And more……… |
more……… |
40g/L |
more….. |
|
YGM003A-N |
Elements Minus/selective |
200g/L |
2440-2520 |
|
YGM003C-1 |
3-AT |
5ml sterile |
|
|
YGM003C-2 |
5-FOA |
250/500mg |
|
|
YGM003D-1 |
40%无菌葡萄糖 |
Discontinued |
|
|
YGM002D-2 |
40%无菌半乳糖 |
50ml |
|
|
YGM003D-3 |
40%无菌棉籽糖（棉子糖） |
50ml |
|
|
YGM011 |
Yeast Transformation Kit |
20T/Kit |
|
|
YGM012 |
Yeast Plasmid Isolation Kit |
20T/Kit |
|
|
YGM013 |
Yeast Protein Isolation Kit |
20T/Kit |
|
|
YGM022A |
10xLiAc无菌 |
20ml |
|
|
YGM022B |
50%PEG无菌 |
20ml |
|
|
YGM022C |
Carrier DNA 运载DNA 酵母转化 |
20ml |
|
|
YGM022D |
10xTE 无菌 |
20ml |
|
|
YGM022E |
Beta-Gal |
20mg/ml |
|
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- 2021-03-31
- 2020-07-31
- 2019-09-16
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
-
- 2021-03-31
- 2020-07-31
- 2019-09-16
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
-
- 2021-03-31
- 2020-07-31
- 2019-09-16
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
