分子遗传学考题重复变换与易错题集

1. DNA binding site is refered to consensus site

2. [ T  or F ]        A consensus site is typically the highest affinity binding site for a transcription factor

3. [ T  or F ]        Transcription factors often drive cell fate decisions

4. Transcription factors determine chromatin structure

5.[T or  F ]        Transcription factors cannot unwind DNA locally.

6. Histone methylation is related to both repression and activation of transcription, though in many cases is involved in repression

7.   Allostery   is the process by which the binding of a small molecule changes the shape of a protein

8. DNA allostery is the process by which the binding of a specific DNA

   sequence changes the shape of a protein

9.  DNA can serve as an allosteric effector

10. Allosteric small molecules that lead to transcriptional repression are called __agonist (这一题容易出错)

11.Partial antagonists   repress   transcription at low concentrations but    

   activate at high concentrations

12.As the concentration of a partial agonist is increased the compound

   switches from inducing transcription to repressing. (True  or False)

13. In prokaryotes, DNA that preferentially curves is bound by Histone-like

    proteins.

14.. Prokaryotes package their genomes using   histone-like proteins  

15. ____CpG islands_____ are the DNA sites often methylated upstream of a promoter

16. Draw RNA polymerase for bacteria showing each subunit clearly and label each subunit appropriately (要熟悉其组成)

17. Draw a picture of a typical eukaryotic nucleosome showing each subunit and label each subunit appropriately including how many of each subunit are found in a nucleosome（要熟悉其组成）

18. TFIIH has kinase activity that contributes to the phosphorylation of the CTD

19. Bending of the DNA at a promoter positively influences transcription by

   increasing  the amount of protein surface area available for protein-

   protein interactions  

20. DNA looping occurs when a transcription factor bind 300 bases away from

   a transcription start site

21. [这一题我都不知道] In budding yeast the primary sequence of DNA helps position nucleosomes across the genome because it favors  curvature of the DNA 

22. [ T or F]        The chromatin immunoprecipitation assay only detects proteins that directly contact         DNA

23. [ T or F ] ChIP cannot be used to measure promoter occupancy of

   proteins that do not bind directly to the DNA

24. [T  or F]        ChIP The chromatin immunoprecipitation assay is used to

   detect proteins that cannot directly bind DNA

25. ChIP-Seq can be used to measure the global occupancy of a protein along a genome

26. Competition between biological factors is a means of regulation

27. RNAP pausing is used to regulate gene expression

8注意：有人在做Western blot时，用代表抗体，例如：-Ser 5, -histone 分别表示抗Ser 5的抗体和抗Histone的抗体

29. A heterodimer binding site represents a composite response element

30. When a transcription factor regulates gene expression by associating

    with the promoter without directly contacting the DNA the response

    element is a Simple.

31 . Simple response element is the term used to describe a DNA binding

     element that is bound by two copies of a transcription factor homodimer

32. Chromatin is composed of just DNA and histone proteins.

（True or False ） 注意：这一题不小心就会答错而选True

33. Gene regulatory mechanisms are generally conserved between

    prokaryotes and eukaryotes.（True or False )

34. Prokaryotes and eukaryotes share few common fundamental principles

    in terms of events that are involved in gene regulation(True or False)

35.  Cholesterol forms the backbone of   cortisol   which can be a potent anti-inflammatory small molecule

36. Cholesterol is the backbone molecule for all steroid hormones.

37. The 4 types of sequence elements used by general transcription factors

     in eukaryotes to bind to a promoter are   TATA box  ,    BRE   , INR   , and    DPE   

38. Tethering element is a response element that binds 2 or transcription

    factors using a single DNA bound protein.