酵母细胞表型(phenotype)-基因型(genotype)

常用酵母细胞表型(phenotype)-基因型(genotype)

Y187 MATα ura3-52, his3-200, ade2-101, trp1-901,leu2-3, 112, gal4Δ, met–, gal80Δ,

MEL1,URA3::GAL1UAS - GAL1TATA-lacZ

AH109 MATa, trp1-901, leu2-3, 112, ura3-52, his3-200,gal4Δ, gal80Δ,

LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2,

URA3::MEL1UAS-MEL1TATA-lacZ, MEL1

YPH499 MATa: ade2-101ochre his3-△200 leu-△1 lys2-801amber trp1△1-63 ura3-52

YHP500 MATα: ade2-101ochre his3-△200 leu-△1 lys2-801amber trp1△1-63 ura3-52

W303-1A MATa: leu2-3, -112; his3-11, -15; trp1-1; ura3-1; ade2-1; can1-100

W303-1B MATα: ade2-1 leu2-3,112 his311 his3-15 trp1-1 ura3-1)

BY4741 MATa:his3?1 leu2?0 met15?0 ura3?0

BY4742 MATα:his3?1 leu2?0 met15?0 ura3?0

YM4271 MATa, ura3-52, his3-200, ade2-101, lys2-801, leu2-3, 112, trp1-901,

tyr1-501, gal4-D512, gal80-D538, ade5::hisG （酵母单杂交系统报告菌株）

JRY2726 MATa:his4

JRY2728 MATα:his4

FGN2749 蛋白酶缺陷株-----用于易降解或不稳定蛋白的高效表达与纯化

MaV203 MATα: leu2-3,112, trp1-901, his3Δ200, ade2-101, gal4Δ, gal80Δ,

SPAL10::URA3, GAL1::lacZ, HIS3UAS GAL1::HIS3@LYS2, can1R, cyh2R) (Vidal, 1997)

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S288C

Genotype: MATα SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.

The S288C genome was recently resequenced at the Sanger Institute.

YPH499

Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker. gal2-, does not use galactose anaerobically.

YPH500

Genotype: MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes:MATα strain isogenic to YPH499 except at mating type locus.

YPH501

Genotype: MATa/MATα ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1- Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1

Notes: a/α diploid isogenic to YPH499 and YPH500.

W303-1A

Genotype: MATa {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.

W303-1B

Genotype: MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

W303

Genotype: MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]

Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variant of MIP1 which increases petite frequency.

The W303 genome was sequenced at the Sanger Institute

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