酵母第二代培养基YGM003A系列及Ura Minus Media Sc-U系列 2022版
酵母选择营养缺陷培养基 &-Sc-U Ura minus Media
酵母第二代培养基系『泛基诺』首创,在泛基诺公司第二代产品上市8年后国外才有同类产品问世。酵母第二代培养基原本为了方便用户配制而生,后来针对不同的酵母实验室在培养不同基因型的酵母时出现生长延迟的情况或不通用的现象,泛基诺公司技术部利用缺陷配对增强专利技术对一般实验用的酵母培养基根据缺陷成分的变化进行调整,设计了各种选择缺陷培养基,适用于各类酵母遗传学实验。
YGM003A系列酵母选择营养缺陷培养基-& Ura Minus Media
【液体培养基】 8g粉状培养基泛基诺目录号YGM0003A系列选择培养基加950 ml 水,常规高压灭菌后再加50ml 40%过滤除菌的无菌葡萄糖。(备注: YPD、YPDA和YPD plus这三种系列不需要再加糖,直接按瓶子上标签指示加水配制即可)。配制液体培养基不需要调pH,因为酵母偏好酸性条件生长。如果为了实验方便,葡萄糖也可按2%的量以粉末的形式加进去高压灭菌(见下面标准配制法注解)。
【固体培养基】8g粉状培养基(泛基诺目录号:YGM0003A系列)加950 ml, 用NaOH调pH至大致范围5.8~6.5, 然后按20g/L加琼脂粉, 高压灭菌后再加预温的50ml 40%无菌葡萄糖, 摇匀后倾倒平皿(备注:如不调pH, 琼脂在酸性条件下会产生崩解)。如果为了实验方便,葡萄糖也可按2%的量以粉末的形式加进去高压灭菌。
【标准配制法】标准流程要求葡萄糖过滤除菌,因为葡萄糖与培养基中的氨基酸在高温高压下发生化学反应。但有些实验者为方便起见,将固体葡萄糖按2% 的量(按每升20克)直接加到培养基后一起高压灭菌,虽然酵母也可以生长,但一般不推荐这种将葡萄糖高压灭菌的方法。如果实在要图省事想用这种方法,那么一定要控制好高压时间在18-20分钟结束,待压力下降和温度降到安全范围后应尽快取出。否则液体颜色会变成黄色而对酵母特性发生不可预知的影响。但半乳糖和棉子糖不能高压灭菌,只能用过滤除菌制备,建议制备高浓度40%的储存液,按计算好的体积加到高压灭菌的培养基使得终浓度为2%。40%无菌棉子糖和半乳糖可以通过当地经销商从泛基诺公司订购;如客户自行配制高浓度半乳糖时发现很难溶解,可通过QQ技术咨询,实时在线工作QQ号:2827505022
【说明】用于组成型表达(如酵母双杂交、酵母单杂交)以及其它采用组成型启动子的实验一般都加葡萄糖;诱导型表达如采用GAL1启动子载体的表达一般都加半乳糖和棉子糖。葡萄糖可以勉强高压灭菌,但半乳糖和棉子糖是绝对不能高压灭菌的, 应采用无菌过滤法先制备40%的无菌储备液,待不含糖的培养基高压灭菌后按最终应用浓度为2% 的量计算需要加入的无菌液体糖储存液:
【举例】如配制1升培养基则称取8克培养基加水950 ml ,常规高压灭菌(121度20分钟)完成后待温度降至安全范围70度时趁热立刻加入50ml 40%过滤除菌的无菌糖,充分混匀。
YGM003A-X系列酵母选择营养缺陷培养基2022
货号 |
品名 |
包装 |
价格 |
YGM003A-1 |
SC Complete medium |
40g(8g/L) |
640.00 |
YGM003A-2 |
-Trp |
40g(8g/L) |
640.00 |
YGM003A-3 |
-Ura |
40g(8g/L) |
640.00 |
YGM003A-4 |
-His |
40g(8g/L) |
640.00 |
YGM003A-5 |
-Leu |
40g(8g/L) |
640.00 |
YGM003A-6 |
-His-Leu |
40g(8g/L) |
640.00 |
YGM003A-7 |
-Trp-Leu |
40g(8g/L) |
640.00 |
YGM003A-8 |
-Trp-Leu-His |
40g(8g/L) |
640.00 |
YGM003A-9 |
-Trp-Leu-His-Ade |
40g(8g/L) |
640.00 |
YGM003A-10 |
-Trp-Leu-His-Ura |
40g(8g/L) |
640.00 |
YGM003A-11 |
-Trp-Leu-His-Ura-Ade |
40g(8g/L) |
640.00 |
YGM003A-12 |
-Trp-Ade |
40g(8g/L) |
640.00 |
YGM003A-13 |
-Ura-His |
40g(8g/L) |
640.00 |
YGM003A-14 |
-Ade |
40g(8g/L) |
640.00 |
YGM003A-15 |
-Trp-His-Ura |
40g(8g/L) |
640.00 |
YGM003A-16 |
-Leu-His-Ura |
40g(8g/L) |
640.00 |
YGM003A-17 |
-Trp-His |
40g(8g/L) |
640.00 |
YGM003A-18 |
-Trp-His-Ade |
40g(8g/L) |
640.00 |
YGM003A-19 |
-Trp-Leu-Ura |
40g(8g/L) |
640.00 |
YGM003A-20 |
-Trp-Leu-Ade |
40g(8g/L) |
640.00 |
YGM003A-21 |
-Leu-His-Ade |
40g(8g/L) |
640.00 |
YGM003A-22 |
-Ura-Ade |
40g(8g/L) |
640.00 |
YGM003A-23 |
-His-Ade |
40g(8g/L) |
640.00 |
YGM003A-24 |
-Leu-Ade |
40g(8g/L) |
640.00 |
YGM003A-31 |
-Trp-Ura |
40g(8g/L) |
640.00 |
YGM003A-32 |
-Trp-Ura-Ade |
40g(8g/L) |
640.00 |
YGM003A-35 |
-Leu-Ura |
40g(8g/L) |
640.00 |
YGM003A-36 |
-Leu-Ura-Ade |
40g(8g/L) |
640.00 |
YGM003A-40 |
-Trp-Leu-His-Ade-Ura |
40g(8g/L) |
640.00 |
YGM003A-50 |
YPD |
250g(50g/L) |
520.00 |
YGM003A-51 |
YPD plus |
250g(50g/L) |
520.00 |
YGM003A-52 |
YPDA |
250g(50g/L) |
520.00 |
YGM003A-60 |
-Trp-Leu-His-Ade-Ura-Met |
40g(8g/L) |
650.00 |
YGM003A-60Lys |
-Trp-Leu-His-Ade-Ura-Met-Lys |
40g(8g/L) |
750.00 |
YGM003A-61 |
-Met |
40g(8g/L) |
650.00 |
YGM003A-61T |
-Met-Trp |
40g(8g/L) |
650.00 |
YGM003A-61L |
-Met-Leu |
40g(8g/L) |
650.00 |
YGM003A-61H |
-Met-His |
40g(8g/L) |
650.00 |
YGM003A-61A |
-Met-Ade |
40g(8g/L) |
650.00 |
YGM003A-61U |
-Met-Ura |
40g(8g/L) |
650.00 |
YGM003A-61C |
-Met-Cys |
40g(8g/L) |
650.00 |
YGM003A-61LC |
-Met-Leu-Cys |
40g(8g/L) |
750.00 |
YGM003A-62UC |
-Met-Ura-Cys |
40g(8g/L |
750.00 |
YGM003A-62TL |
-Met-Trp-Leu |
40g(8g/L) |
650.00 |
YGM003A-62TH |
-Met-Trp-His |
40g(8g/L) |
650.00 |
YGM003A-62TA |
-Met-Trp-Ade |
40g(8g/L) |
650.00 |
YGM003A-62TU |
-Met-Trp-Ura |
40g(8g/L) |
650.00 |
YGM003A-62LH |
-Met-Leu-His |
40g(8g/L) |
650.00 |
YGM003A-62LA |
-Met-Leu-Ade |
40g(8g/L) |
650.00 |
YGM003A-62LU |
-Met-Leu-Ura |
40g(8g/L) |
650.00 |
YGM003A-62UA |
-Met-Ura-Ade |
40g(8g/L) |
650.00 |
YGM003A-63TLH |
-Met-Trp-Leu-His |
40g(8g/L) |
650.00 |
YGM003A-63TLA |
-Met-Trp-Leu-Ade |
40g(8g/L) |
650.00 |
YGM003A-63LHA |
-Met-Leu-His-Ade |
40g(8g/L) |
650.00 |
YGM003A-63LHU |
-Met-Leu-His-Ura |
40g(8g/L) |
650.00 |
YGM003A-63HAU |
-Met-His-Ade-Ura |
40g(8g/L) |
650.00 |
YGM003A-64TLHA |
-Met-Trp-Leu-His-Ade |
40g(8g/L) |
650.00 |
YGM003A-64TLHU |
-Met-Trp-Leu-His-Ura |
40g(8g/L) |
650.00 |
YGM003A-64TLAU |
-Met-Trp-Leu-Ade-Ura |
40g(8g/L) |
650.00 |
YGM003A-64LHAU |
-Met-Leu-His-Ade-Ura |
40g(8g/L) |
650.00 |
YGM003A-64THAU |
-Met-Trp-His-Ade-Ura |
40g(8g/L) |
650.00 |
YGM003A-65 |
-Lys |
40g(8g/L) |
750.00 |
YGM003A-65TLHUC |
-Met-Trp-Leu-His-Ura-Cyc |
40g(8g/L) |
750.00 |
YGM003A-66 |
-Arg |
40g(8g/L) |
750.00 |
YGM003A-67 |
-Ser |
40g(8g/L) |
750.00 |
YGM003A-68 |
-Thr |
40g(8g/L) |
750.00 |
YGM003A-69 |
-Lys-Arg-Ser-Thr |
40g(8g/L) |
750.00 |
YGM003A-70 |
-Arg-Lys |
40g(8g/L) |
750.00 |
YGM003A-71 |
-Ser-Thr |
40g(8g/L) |
750.00 |
YGM003A-72 |
-Arg-His-Leu-Ura |
40g(8g/L) |
750.00 |
YGM003A-73 |
-Lys -Trp |
40g(8g/L) |
750.00 |
YGM003A-74 |
-Arg-His-Leu |
40g(8g/L) |
750.00 |
YGM003A-75 |
-Arg-Leu-Ura |
40g(8g/L) |
750.00 |
YGM003A-76 |
-Lys-Ura |
40g(8g/L) |
750.00 |
YGM003A-77 |
-Ile-Val |
40g(8g/L) |
750.00 |
YGM003A-78 |
-Val |
40g(8g/L) |
750.00 |
YGM003A-79 |
-Ile |
40g(8g/L) |
750.00 |
YGM003A-80 |
-Ile-Val-Leu |
40g(8g/L) |
750.00 |
YGM003A-81 |
-Tyr |
40g(8g/L) |
750.00 |
YGM003A-82 |
-Asp |
40g(8g/L) |
750.00 |
YGM003A-83 |
-Phe |
40g(8g/L) |
750.00 |
YGM003A-84 |
-Arg-Ura-His |
40g(8g/L) |
750.00 |
YGM003A-85 |
-Arg-Leu |
40g(8g/L) |
750.00 |
YGM003A-86 |
-Ser-Trp-Leu |
40g(8g/L) |
750.00 |
YGM003A-87 |
-Lys-Leu-Ura |
40g(8g/L) |
750.00 |
YGM003A-88 |
-Lys-Arg-Leu-Ura-His |
40g(8g/L) |
750.00 |
YGM003A-89 |
-Lys-Leu-Ura-His |
40g(8g/L) |
750.00 |
YGM003A-90 |
-Lys-His |
40g(8g/L) |
750.00 |
YGM003A-91 |
-Ser-Trp-Leu-Ade |
40g(8g/L) |
750.00 |
YGM003A-92 |
-Lys-Ura-His |
40g(8g/L) |
750.00 |
YGM003A-93 |
+Leu+His-All |
40g(8g/L) |
750.00 |
YGM003A-94 |
-Arg-Ura |
40g(8g/L) |
750.00 |
YGM003A-95 |
-Lys-Leu-His |
40g(8g/L) |
750.00 |
YGM003A-96 |
-Cys |
40g(8g/L) |
750.00 |
YGM003A-97 |
-Lys-Leu |
40g(8g/L) |
750.00 |
YGM003A-98 |
-Ala-Cys-Asn-Gln-Pro-Ser |
40g(8g/L) |
750.00 |
YGM003A-99 |
-Lys-Trp-Leu-His-Ura |
40g(8g/L) |
750.00 |
more……… |
more……… |
more…… |
more….. |
YGM011 |
Yeast Transformation Kit |
20T/Kit |
000.00 |
YGM012 |
Yeast Plasmid Isolation Kit |
20T/Kit |
000.00 |
YGM013 |
Yeast Protein Isolation Kit |
20T/Kit |
000.00 |
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- 2021-03-31
- 2020-07-31
- 2019-09-16
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
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- 2021-03-31
- 2020-07-31
- 2019-09-16
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
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- 2021-03-31
- 2020-07-31
- 2019-09-16
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29
- 2019-08-29