《Nature》史上引用次数最多的十篇论文

                              《Nature》史上引用次数最多的十篇论文

《Nature》杂志上发表了一篇关于史上引用次数最多的论文排序，其中生物研究类占据很大的范围；目前引用最多的文献是1951年的论文，描述了如何计算溶液中蛋白的含量，它已经有305000次引用。下面是引用前十大Nature论文：

　　1：protein measurement with the folin phenol reagent(1951);引用次数：305,148

　　2：Cleavage of structural proteins during the assembly of head of the dacteriophage T4(1970);引用次数：213,005

　　3：A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding(1976);引用次数：155,530

　　4：DNA sequencing with chain-terminating inhibitors(1977);引用次数：65,335

　　5：Singl-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction(1987);引用次数：60,397

　　6：Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets:procedure and some applications(1979)引用次数：53,349

　　7：Deveiopment of the colle-Salvetti correlation-energy formula into a functional of the electron density(1988);引用次数：46,702

　　8：Density-functional thermochemistry. lll. The role of exact exchange(1993);引用次数：46,145

　　9：A simple method for the isolation and purification of total lipides from animal tissues(1957);引用次数：45,131

　　10：CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment therough sequence weighting,position-sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice(1994);引用次数：40,289

　　Just 3 papers have received more than 100000 citations, putting them well ahead of the rest.these runaway tits all cover biological lab techniques,which in general dominate the list of most-cited literature, including 7 of the top 10.

此次引用前100的文章，是从1900年开始计算的。要进入前100名，至少要引用12119次！奇怪的是很多有名的论文都没有进入前100，如高温超导的发现，DNA双螺旋的结构确定，以及首次观察到宇宙扩张速度的加快等重大发现，这些都获得过诺贝尔奖，可是相关的论文都没有进入引用前100的论文。